VZV PCR Kit

Description

• Excellent Sensitivity
• Internal Control guarantees High Reliability
• Easy-to-use Real-Time PCR Kit

The VZV PCR Kit constitutes a ready-to-use system for the detection of VZV DNA using Real-Time polymerase chain reaction (PCR)in the ABI PRISM® 7000 Sequence Detection System (Applied Biosystems).  The kit contains reagents and enzymes for the specific amplification of a 82 bp region of the VZV genome.  The amplicon is detected by measuring the FAM fluorescence in the ABI PRISM® SDS.  In addition, the VZV PCR Kit contains a second heterologous amplification system to identify possible PCR inhibition.  This is detected as an Internal Control (IC) by measuring VIC fluorescence.  External positive controls are supplied which allow the determination of the pathogen load.

CE label
The VCV PCR Kit is CE marked according to the European IVD Directive 98/79/EC

Excellent Sensitivity
VCV PCR Kit has been optimized to detect very low viral load down to 0.4 copies/µl.

Specificity
The specificity of the VZV PCR Kit is first and foremost ensured by the selection of the primers and probes, as well as the selection of stringent reaction conditions. The detectability of all relevant strains has thus been ensured.

Internal Control
The Internal Control allows the user both to control the nucleic acid isolation efficiency and to check for possible PCR inhibition.  Optionally it can be used exclusively to check for possible PCR inhibition.

Quantitative Results
External Quantitation Standards of different concentrations (QS 1-4) are supplied for precise quantitation of the pathogen load.

Flexible Throughput
The instrument allows the handling of one sample up to 96 samples in one single run.

Easy-to-use Real-Time PCR Kit 

Manual Sample Preparation		Preparation of PCR Reaction		Amplification, Detection & Results
30 Minutes		15 Minutes		120 Minutes