TriGenTM

20286_3765
TriGen

Product Description

Probe Name Probe Location Fluorophore
Vysis® LSI® 21 21q22.13-22.2  SpectrumOrange TM
Vysis CEP® X Xp11.1-q11.1  SpectrumGreen TM
Vysis CEP Y Yp11.1-q11.1 Alpha Satellite DNA SpectrumOrange TM
The TriGenTM Assay is a fast, reliable and simple test kit for detection of trisomy 21 and aneusomy of chromosomes X and Y. In clinical trials, the TriGen Assay detected trisomy 21 and aneusomy of chromosomes X and Y within 6 hours with 100% accuracy. The TriGen Assay provides definitive diagnostic information from direct amniocyte preparations. The test fits easily into the routine of the cytogenetic laboratory. The TriGen Assay kit provides an easy and economical way to perform standardized assays. Each kit contains the essential components needed to perform 20 standardized assays (22 x 22 mm area). TriGen is registered as an in vitro diagnostic product with the French AFSSAPS (Registration No. R84932). The TriGen Assay is only available for sale outside of the United States.

Probe Map



TriGen Kit Components
3 DNA Probes

LSI 21 SpectrumOrange DNA FISH Probe

  • Contains unique DNA sequences complementary to the loci D21S259, D21S341 and D21S342 contained within the 21q22.13-21q22.2 region on the long arm of chromosome 21 and is approximately 200 kb in size. LSI 21 probe is directly labeled with the SpectrumOrange fluorophore.
CEP X SpectrumGreen and CEP Y SpectrumOrange DNA FISH Probes

  • Contain chromosome-specific alphoid DNA sequences. The CEP X probe hybridizes to the centromere region of chromosome X (Xp11.1-q11.1). CEP X probe is directly labeled with the SpectrumGreen fluorophore. The CEP Y probe hybridizes to the centromere region of chromosome Y (Yp11.1-q11.1). CEP Y probe is directly labeled with the SpectrumOrange fluorophore.
Positive and Negative Controls

ProbeChekTM Positive and Negative Control Slides

  • Confirm that the assay is performing within expected parameters. Can be used in training technologists to interpret FISH on interphase cells.
  • The TriGen Assay Kit contains 5 positive and 5 negative control slides.
Reagents

20X SSC (For preparation of denaturation and wash solutions)
NP-40 (For preparation of wash solutions) DAPI II Counterstain (Used to counterstain the cells)
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Order Information

Product Name Package Size Vysis Order Number Abbott Order Number
TriGen  20 Assays 30-161040 05J40-001
For In Vitro Diagnostic Use.