Single Bandpass Filter Sets

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Single Bandpass Filter Sets

Orange
The Orange filter set is designed to excite and transmit SpectrumOrange™ fluorescence. All of the Vysis® SpectrumOrangeTM labeled DNA FISH probes can be viewed, analyzed, and imaged using this filter set. The SpectrumRed™ fluorophore will be visible using this filter set, but will be dim.

Red
The Red filter set is designed to excite and transmit SpectrumRedTM fluorescence. This filter set can be used to view, analyze, and image SpectrumRed labeled probes, or for TexasRed labeled DNA FISH probes that are available from other probe manufacturers.
The SpectrumRed filter set is not recommended for viewing SpectrumOrange labeled probes.

Yellow
The Yellow filter set is designed to excite and transmit SpectrumGold™ fluorescence. The Vysis SpectrumGoldTM labeled DNA FISH probes (Vysis UroVysion™) can be viewed, analyzed, and imaged using this filter set. SpectrumOrange probe fluorescence
will also be visible with the Yellow filter set. SpectrumRed fluorescence may be visible, yet very dim.

Green
The Green filter set is designed to excite and transmit SpectrumGreen™ fluorescence. This filter set can be used to view, analyze, and image SpectrumGreenTM and fluorescence labeled DNA FISH probes.

Aqua
The Aqua filter set is designed to excite and transmit SpectrumAqua™ fluorescence.  This filter set is specifically required for viewing, analyzing, and imaging Vysis SpectrumAquaTM DNA probes. Filter sets that excite and transmit DAPI fluorescence
are not appropriate for SpectrumAqua labeled probes. In some instances,when hybridization signal is very intense for the SpectrumAqua labeled DNA probe, the aqua fluorescence may be visible through a DAPI filter set.However, this will not provide a
reliable method for analysis of SpectrumAqua labeled probes. In addition, on specimens counterstained with DAPI, extremely weak DAPI fluorescence may be observed when viewing or imaging through an Aqua single bandpass filter set.

Blue
The Blue filter set is designed to excite and transmit SpectrumBlue™ fluorescence and is useful when viewing the SpectrumBlueTM fluorophore alone.DAPI fluorescence will also be visible with this filter set. SpectrumAqua fluorescence will be visible through the
Blue filter set, but will be dim. In order to view the SpectrumBlue and SpectrumAqua fluorophores simultaneously, the Aqua/Blue dual bandpass filter set should be used.

DAPI Longpass
The DAPI longpass filter set is designed to excite and transmit DAPI counterstain fluorescence. This filter set transmits more in the red range of the color spectrum than the DAPI bandpass filter set.

DAPI Bandpass
The DAPI bandpass filter set is designed to excite and transmit DAPI counterstain. This filter set does not transmit as much of the spectrum as the DAPI Longpass filter set, and is recommended over the DAPI Longpass for imaging applications.