Vysis® ProVysionTM Multi-color Probe
Product Description
| Probe Name | Probe Location | Fluorophore |
| Vysis LSI® LPL | 8p22 | SpectrumOrange TM |
| Vysis LSI C-MYC | 8q24.12-24.13 | SpectrumGreen TM |
| Vysis CEP® 8 | 8p11.1-q11.1 Alpha Satellite DNA | SpectrumAqua TM |
The ProVysion Multi-color Probe is designed to detect and quantify chromosome 8, the lipoprotein lipase (LPL) gene located at 8p22, and the C-MYC gene located at the 8q24 region. Two genetic alterations that have been observed in abnormal samples are gain of 8q24 and 8p21-22 (LPL) loss of heterozygosity. The ProVysion Multi-color Probe consists of three probes with three separate fluorophore labels. The multi-color probe design allows simultaneous analysis of the three genomic markers within a single cell.
The ProVysion Multi-color Probe consists of the CEP 8 probe labeled with SpectrumAqua, LSI LPL labeled with SpectrumOrange, and LSI C-MYC labeled with SpectrumGreen. The CEP 8 alpha satellite DNA probe hybridizes to the centromere region of chromosome 8 (8p11.1-q11.1) and provides a mechanism for the identification of copy number of chromosome 8. The LSI LPL hybridizes to the LPL gene at 8p22 and is approximately 170 kb in size. The LSI C-MYC Probe hybridizes to the C-MYC gene located at 8q24 and is an approximately 750 kb probe. This probe set is premixed in Hybridization Buffer.
The ProVysion Multi-color Probe consists of the CEP 8 probe labeled with SpectrumAqua, LSI LPL labeled with SpectrumOrange, and LSI C-MYC labeled with SpectrumGreen. The CEP 8 alpha satellite DNA probe hybridizes to the centromere region of chromosome 8 (8p11.1-q11.1) and provides a mechanism for the identification of copy number of chromosome 8. The LSI LPL hybridizes to the LPL gene at 8p22 and is approximately 170 kb in size. The LSI C-MYC Probe hybridizes to the C-MYC gene located at 8q24 and is an approximately 750 kb probe. This probe set is premixed in Hybridization Buffer.
Probe Map
Results of Hybridization
In a normal cell hybridized with the ProVysion Multi-color Probe, the expected pattern is the two orange, two green and two aqua (2O2G2A) signal pattern. In an abnormal cell, combinations of copies of the three probe signals may be observed.Copy numbers of more or less than two of any probe indicates chromosome or gene gain or loss, respectively. Less than two copies of the LSI LPL or multiple copies of the LSI CMYC Probe relative to CEP 8 copy number indicates loss of the LPL region and gain of the C-MYC region, respectively, relative to the chromosome 8 copy number.
ProVysion Multi-color Probe hybridized to an abnormal cell showing abnormal copy number of chromosome 8 and C-MYC. CEP 8 (aqua) is present in four copies, C-MYC (green) is present in four copies and LPL (orange) is present in two copies (4A4G2O).
References
- Bova, G.S., et al. (1993) Cancer Res 53, 3869-73.
- Cher, M.L., et al. (1994) Genes Chrom Cancer 11, 153-62.
- Emmert-Buck, M.R., et al. (1995) Cancer Res 55, 2959-62.
- Jenkins, R.B., et al. (1997) Cancer Res 57, 524-31.
- Kagan, J., et al. (1995) Oncogene 11, 2121-6.
- Sato, K., et al. (1999) J Natl Cancer Inst 91, 1574-80.
- Shima-Rich, E.A., et al. (1997) Genes Chrom Cancer 20, 363-71.
- Visakorpi, T., et al. (1995) Cancer Res 55, 342-7.
Analyte Specific Reagent.
Analytical and performance characteristics are not established.
Analytical and performance characteristics are not established.
