Vysis® LSI® p53 / LSI ATM and LSI D13S319 / LSI 13q34 / CEP® 12 Multi-color Probe

5611_6294
LSI p53/LSI ATM and LSI D13S319/LSI 13q34/CEP 12 Multi-color Probe

Product Description

Probe Name Probe Location Fluorophore
Vysis LSI p53 17p13.1  SpectrumOrange TM
Vysis LSI ATM 11q22.3  SpectrumGreen TM
Vysis LSI S13S319 13q14.3  SpectrumOrange TM
Vysis LSI 13q34 13q34  SpectrumAqua TM
Vysis CEP 12 12p11.1-q11  SpectrumGreen TM
The leukemias are a diverse group of diseases that are often characterized by multiple genetic aberrations distributed across the genome. In some cases, the same genetic aberrations are shared by different types of leukemia. Trisomy 12 and deletions of chromosomes 13q (primarily the 13q14 region), 17p13.1, and 11q22.32-4, for example, have all been observed in several types of leukemia. Probe vial 1 contains LSI p53 in SpectrumOrange and LSI ATM in SpectrumGreen. Probe vial 2 contains LSI D13S319 in SpectrumOrange, LSI 13q34 in SpectrumAqua, and CEP 12 in SpectrumGreen.

The LSI p53 (17p13.1) probe is a ~145 kb unique sequence probe that is labeled in SpectrumOrange.

The LSI ATM probe is a ~500 kb unique sequence probe that hybridizes to the 11q22.3 region of chromosome 11. This probe spans the entire ~184 kb Ataxia telangiectasia mutated (ATM) gene and several others. The probe is labeled in SpectrumGreen.

The LSI D13S319 probe is a ~130 kb unique sequence probe that is labeled in SpectrumOrange.

The LSI 13q34 probe is a ~550 kb unique sequence probe that hybridizes to the 13q34 region containing the Lysosomal-associate Membrane Protein (LAMP1) gene and several others. The probe is labeled in SpectrumAqua.

The CEP 12 DNA probe hybridizes to the alpha satellite (centromeric) region (12p11.1-q11) of chromosome 12. The probe is labeled in SpectrumGreen.

Probe Map




 


Results of Hybridization

This multi-color probe set is provided in a two-mixture format. A description of the hybridization results expected with each mixture follows:

Probe 1:
This probe allows status assessment of the following two chromosome regions: 17p13.1 (p53) and 11q22.3 (ATM). In a normal cell with two intact copies of chromosomes 17 and 11, two orange, and two green signals will be observed. In an abnormal cell with a deletion in the p53 region, fewer than two orange signals will be observed. In an abnormal cell with a deletion in the ATM region on chromosome 11, one will observe fewer than two green signals.

Probe 2:
This probe allows status assessment of the following three chromosome regions:
13q14.3 (D13S319), 13q34, and 12p11.1-q11. In a normal cell with two intact copies of chromosome 13 and chromosome 12, a two orange, two aqua, and two green signal pattern will be observed. In an abnormal cell with chromosome 13 aberrations only, more complex signal patterns may be expected depending upon the nature of the aberration.  Monosomy 13 or 13q- will both appear as a one orange, one aqua, two green signal pattern.  An interstitial deletion of the 13q14.3 region will appear as either a one orange, two aqua, two green signal pattern (hemizygous deletion) or a two aqua, two green signal pattern (homozygous deletion) (data not shown). In an abnormal cell with chromosome 12 copy number changes, one will observe greater or less than two green signals.

An abnormal cell hybridized with Probe Set 1. One copy of the p53 region has been deleted as indicated by the single orange signal. Both copies of the ATM gene region are present as indicated by the two green signals.
An abnormal cell hybridized with Probe Set 1. One copy of the ATM gene region has been deleted as indicated by the single green signal. Both copies of the p53 gene region are present as indicated by the two orange signals.
An abnormal cell hybridized with Probe Set 2. Both copies of chromosome thirteen and its q arm are intact as indicated by the two orange (LSI D13S319) and two aqua (LSI 13q34) signals. One extra copy of chromosome 12 (trisomy 12) is present as indicated by the three green signals.
 
An abnormal cell hybridized with Probe Set 2. One copy of chromosome 13 is deleted for the D13S319 region as indicated by the single orange signal (LSI D13S319) and the two aqua signals (LSI 13q34). One extra copy of chromosome 12 (trisomy 12) is present as indicated by the three green signals.

References

  1. Anastasi, J., et al. Blood 79 (1992): 1796-1801.
  2. Dohner, H., et al. New Eng Jour Med. 343 (2000): 1910-1916.
  3. Bennarji, R., Byrd, J.C. Curr. Opin Oncol 12 (2000): 22-29.
  4. Stilgenbauer, S., et al. Leukemia 16 (6) (2002): 993-1007.
  5. Pettitt, A.R., et al. Blood 98 (3) (2001): 814-822.
  6. Stankovic, T., et al. Blood 99 (1) (2001): 300-309.
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Order Information

Product Name StatusPackage Size Vysis Order Number Abbott Order Number
Vysis LSI p53/LSI ATM and LSI D13S319/LSI 13q34/CEP 12 Multi-color Probe  ASR 20 µL 32-191025 05J83-001
5J83
Vysis LSI p53/LSI ATM and LSI D13S319/LSI 13q34/CEP 12 Multi-color Probe  20 µL 32-161025 08L53-020
8L53
Analyte Specific Reagent.
Analytical and performance characteristics are not established.

CE Marked Not available in United States