Vysis® LSI® p16 (9p21) SpectrumOrangeTM / CEP® 9 SpectrumGreenTM Probe
Product Description
| Probe Name | Probe Location | Fluorophore |
| Vysis LSI p16 | 9p21 | SpectrumOrange TM |
| Vysis CEP 9 | 9p11-q11 Alpha Satellite DNA | SpectrumGreen TM |
The LSI p16/CEP 9 Dual Color Probe is designed to detect 9p21 deletions. The p16 tumor suppressor gene has been found to lie within the 9p21 region, which is commonly deleted in many malignant disorders.
The LSI p16/CEP 9 Dual Color Probe is a mixture of the LSI p16 probe labeled with SpectrumOrange and the CEP 9 probe labeled with SpectrumGreen. The LSI p16 SpectrumOrange probe spans approximately 190 kb and contains a number of genetic loci including D9S1749, D9S1747, p16(INK4A), p14(ARF), D9S1748, p15(INK4B), and D9S1752. The CEP 9 SpectrumGreen probe hybridizes to alpha satellite sequences specific to chromosome 9.
The LSI p16/CEP 9 Dual Color Probe is a mixture of the LSI p16 probe labeled with SpectrumOrange and the CEP 9 probe labeled with SpectrumGreen. The LSI p16 SpectrumOrange probe spans approximately 190 kb and contains a number of genetic loci including D9S1749, D9S1747, p16(INK4A), p14(ARF), D9S1748, p15(INK4B), and D9S1752. The CEP 9 SpectrumGreen probe hybridizes to alpha satellite sequences specific to chromosome 9.
Probe Map
Results of Hybridization
In a normal sample, the expected pattern for a nucleus hybridized with the LSI p16/CEP 9 probe is the two orange, two green (2O2G) signal pattern. If a deletion at the 190 kb region covered by the LSI p16 probe occurs on one chromosome 9 homolog and both centromeres from chromosome 9 are retained, the one orange, two green (1O2G) signal pattern is expected. Very small deletions may occur that do not delete the entire LSI p16 probe target and therefore will not be detected.
LSI p16(9p21)/CEP 9 Dual Color Probe hybridized to a nucleus exhibiting the one orange and two green signal (1O2G) pattern. One p16 gene locus is deleted and both chromosome 9 homologs are present as indicated by one orange and two green signals, respectively.
References
- Balazs, M., et al. (1997) Genes Chrom Cancer 19, 84-9.
- Okuda, T., et al. (1995) Blood 85, 2321-30.
- Perry, A., et al. (1997) J Neuropathol Exp Neurol 56, 999-1008.
- Sherr, C., (2000) Cancer Res 60, 3689-95.
- Xiao, S., et al. (1995) Cancer Res 55, 2968-71.
Analyte Specific Reagent.
Analytical and performance characteristics are not established.
Analytical and performance characteristics are not established.
