Vysis® LSI® TOP2A SpectrumOrange/ HER-2 SpectrumGreen/ CEP® 17 SpectrumAquaTM Probe
Product Description
| Probe Name | Probe Location | Fluorophore |
| Vysis LSI TOP2A | 17q21-22 | SpectrumOrange TM |
| Vysis LSI HER-2 | 17q11.2-12 | SpectrumGreen TM |
| Vysis CEP 17 | 17p11.1-q11.1 Alpha Satellite DNA | SpectrumAqua TM |
The TOP2A gene, located at 17q21-q22, encodes topoisomerase II- a key enzyme in DNA replication, cell cycle progression, and chromosome segregation.As a key enzyme in DNA replication, TOP2A protein is the molecular target for many inhibitors. The TOP2A gene is located telomeric to the HER-2 oncogene, which is located in the 17q11.2-q12 region.HER-2 is one member of a family of transmembrane protein receptors. The close proximity of HER-2, TOP2A, and other genes in the 17q region, suggest a potential relationship between these genes. This probe set is premixed in Hybridization Buffer.
LSI TOP2A is a single ~160 kb unique sequence probe that hybridizes to the 17q21-22 region containing the TOP2A gene. In both products, the probe is directly labeled with SpectrumOrange. The HER-2 probe that spans the entire HER-2 gene at 17q11.2-q12 is an ~190 kb unique sequence probe. In the LSI TOP2A/HER-2/CEP 17 product, this probe is directly labeled with SpectrumGreen. The CEP 17 probe, which hybridizes to alpha satellite DNA at 17p11.1-q11.1, is directly labeled with SpectrumGreen or SpectrumAqua in the LSI TOP2A/CEP 17 and LSI TOP2A/HER-2/ CEP 17 products, respectively.
LSI TOP2A is a single ~160 kb unique sequence probe that hybridizes to the 17q21-22 region containing the TOP2A gene. In both products, the probe is directly labeled with SpectrumOrange. The HER-2 probe that spans the entire HER-2 gene at 17q11.2-q12 is an ~190 kb unique sequence probe. In the LSI TOP2A/HER-2/CEP 17 product, this probe is directly labeled with SpectrumGreen. The CEP 17 probe, which hybridizes to alpha satellite DNA at 17p11.1-q11.1, is directly labeled with SpectrumGreen or SpectrumAqua in the LSI TOP2A/CEP 17 and LSI TOP2A/HER-2/ CEP 17 products, respectively.
Probe Map
Results of Hybridization
LSI TOP2A/HER-2/CEP 17 Multi-color Probe
As with TOP2A and chromosome 17, a nucleus with a normal quantity (two copies) of HER-2 will appear with two green signals. Simultaneous enumeration of all three probes will reveal the copy number of each as well as the amplification or deletion status of TOP2A and HER-2 relative to chromosome 17 copy number. The ability to distinguish true gene amplification or deletion from aneusomy of chromosome 17 or nuclei truncation is an added benefit of this multi-color probe.
Result of the hybridization of the LSI TOP2A/HER-2/CEP 17 Multi-color Probe as observed in two normal interphase cells. In the nucleus located in the upper left, two copies of each of the three probes are observed: orange (TOP2A), green (HER-2), and aqua (CEP 17). The lower right nucleus exhibits normal hybridization results for LSI HER-2 and CEP 17 but is lacking one TOP2A signal.
Tumor cells hybridized with the LSI TOP2A/ HER-2/CEP 17 Multi-color probe. The cells in this image show amplification of both TOP2A (orange signals) and HER-2 (green signals) as indicated by multiple signals of each color. Aqua signals from the CEP 17 probe indicate that chromosome 17 is present in the normal quantity (two copies).
References
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Analyte Specific Reagent.
Analytical and performance characteristics are not established.
Analytical and performance characteristics are not established.
