Vysis® LSI® TEL/AML1 ES Dual Color Translocation Probe
Product Description
| Probe Name | Probe Location | Fluorophore |
| Vysis LSI TEL | 12p13 | SpectrumGreen TM |
| Vysis LSI AML1 | 21q22 | SpectrumOrange TM |
The LSI TEL/AML1 ES Dual Color Translocation Probe is designed to detect the TEL (ETV6)/AML1 (RUNX1)gene fusion that occurs as a result of a translocation between chromosomes 12p13 and 21q22. Cytogenetically, the t(12;21) is a subtle abnormality and thus not easily detectable with standard cytogenetic banding techniques.
The LSI TEL/AML1 ES Dual Color Translocation Probe is a mixture of the LSI TEL probe labeled with SpectrumGreen and the LSI AML1 probe labeled with SpectrumOrange. The LSI TEL probe begins between exons 3-5 and extends approximately 350 kb toward the telomere on chromosome 12. The approximately 500 kb AML1 probe spans the entire gene.
The LSI TEL/AML1 ES Dual Color Translocation Probe is a mixture of the LSI TEL probe labeled with SpectrumGreen and the LSI AML1 probe labeled with SpectrumOrange. The LSI TEL probe begins between exons 3-5 and extends approximately 350 kb toward the telomere on chromosome 12. The approximately 500 kb AML1 probe spans the entire gene.
Probe Map
Results of Hybridization
In a normal nucleus, the expected pattern for a cell hybridized with the LSI TEL/AML1 ES Dual Color Translocation probe is the two orange (AML1), two green (TEL) (2O2G) signal pattern. In an abnormal cell containing the TEL/AML1 fusion, the expected signal pattern is one green (native TEL), one large orange (native AML1), one smaller orange signal (residual AML1) and one fused orange/green (yellow) signal. The green native signal may be absent in some instances due to the deletion of the non-translocated TEL allele.
LSI TEL/AML1 ES Dual Color Translocation Probe hybridized to a nucleus lacking the TEL/AML1 fusion gene showing the two orange and two green (2O2G) signal pattern.
References
- Golub, TR. et. al.1995. Proc. Natl. Acad. Sci. USA, 92: 4917-4921;
- Romona, SP. et. al.1995. Blood, 86(110): 4264-4269;
- Shurtleff, SA. et. al. 1995. Leukemia 9: 1985-1989;
- Cayuela, JM, et. al. 1996. Blood, 88(1): 302-308;
- Raynaud, S. et. al. 1996. Blood, 87(7): 2891-2899.
Analyte Specific Reagent.
Analytical and performance characteristics are not established.
Analytical and performance characteristics are not established.
