Vysis® LSI® SYT (18q11.2) Dual Color, Break Apart Rearrangement Probe
Product Description
| Probe Name | Probe Location | Fluorophore |
| Vysis LSI SYT | 18q11.2 | SpectrumGreen TM SpectrumOrange TM |
Hybridization with the LSI SYT (18q11.2) Break Apart Rearrangement Probe will identify t(18q11.2) but not the specific translocation partner.
The LSI SYT Dual Color, Break Apart Rearrangement Probe consists of a mixture of two FISH DNA probes. The first probe, an ~650 kb probe labeled in SpectrumOrange, extends distally from the SYT gene. The second probe labeled in SpectrumGreen lies 3' or proximal to the SYT gene and is approximately ~1040 kb in length.
The LSI SYT Dual Color, Break Apart Rearrangement Probe consists of a mixture of two FISH DNA probes. The first probe, an ~650 kb probe labeled in SpectrumOrange, extends distally from the SYT gene. The second probe labeled in SpectrumGreen lies 3' or proximal to the SYT gene and is approximately ~1040 kb in length.
Probe Map
LSI SYT (18q11.2). The LSI SYT Dual Color Break Apart Rearrangement Probe consists of a mixture of two FISH DNA probes. The first probe, an ~650 KB probe labeled in SpectrumOrange , extends distally from the SYT gene. The second probe labeled in SpectrumGreen lies 3' or proximal to the SYT gene and is approximately ~1040 kb in length.
Results of Hybridization
In a normal cell that lacks a t(18q11.2) in the SYT gene region, a two fusion signal pattern will be observed, reflecting the two intact copies of SYT (Figure 1). In an abnormal cell with a simple t(18q11.2), a one fusion, one green and one orange signal pattern will be expected.
Result of the hybridization of the LSI SYT (18q11.2) Dual Color Break Apart Rearrangement Probe, showing the two fusion signal pattern as observed in normal interphase cells. (Photo courtesy of Arie Perrry, M.D., Washington University School of Medicine.)
Abnormal cells hybridized with the LSI SYT (18q11.2) Dual Color, Break Apart Rearrangement Probe. The cells in this image show the one fusion, one orange and one green signal pattern indicative of a rearrangement of one copy of the SYT gene region. (Photo courtesy of Arie Perry, M.D., Washington University School of Medicine.)
References
- Sandberg A., et al. Cancer Genetics and Cytogenetics 133 (2002): 1-23.
- Dos Santos N., et al.Genes, Chromosomes and Cancer 30 (2001): 1-14.
Analyte Specific Reagent.
Analytical and performance characteristics are not established.
Analytical and performance characteristics are not established.
