Vysis® LSI® RARA Dual Color, Break Apart Rearrangement Probe
Product Description
| Probe Name | Probe Location | Fluorophore |
| Vysis LSI RARA | 17q12-21 | SpectrumOrange TM SpectrumGreen TM |
The LSI RARA Dual Color, Break Apart Rearrangement Probe is used to detect variant rearrangements involving the RARA gene with genes other than PML. This probe may also be used to detect nuclei that have t(15;17) or other variants because of its unique break apart design.
The LSI RARA Dual Color, Break Apart Rearrangement Probe consists of a SpectrumOrange labeled RARA probe that hybridizes to a 130 kb region that extends from the breakpoint cluster region (bcr) toward the centromere and a SpectrumGreen labeled probe that hybridizes to a 400 kb region that begins about 500 bp 3' to exon 6 and extends toward the telomere.
The LSI RARA Dual Color, Break Apart Rearrangement Probe consists of a SpectrumOrange labeled RARA probe that hybridizes to a 130 kb region that extends from the breakpoint cluster region (bcr) toward the centromere and a SpectrumGreen labeled probe that hybridizes to a 400 kb region that begins about 500 bp 3' to exon 6 and extends toward the telomere.
Probe Map
Results of Hybridization
The signal pattern observed in a cell that is lacking a RARA gene rearrangement consists of two orange/green (yellow) fusion signals (2F). The two fusion signals represent the normal (non-rearranged) RARA genes located on both 17 chromosomes. A signal pattern indicative of the RARA gene rearrangement is one orange, one green, and one green/orange (yellow) fusion signal. The separation of orange and green signals from one fusion (1O1G1F) indicates that the RARA gene has split apart. The remaining single fusion signal represents the normal (non-rearranged RARA) gene on the normal chromosome extends approximately 400 kb toward the telomere of chromosome 17.
LSI RARA Dual Color, Break Apart Rearrangement Probe hybridized to nuclei containing the RARA gene rearrangement. A one orange, one green and one fusion (1O1G1F) signal pattern is observed.
References
- Brunel,V., et al. Genes. Chrom. Cancer 4 (1995): 307-12.
- Heim, S., et al. Cancer Cytogenetics 2nd ed. (1995): 377.
- Mozziconacci, M.J., et al. Leukemia 6 (1999): 862-8.
- Wells, R.A., et al. Nat. Genet. 1 (1997): 109-13.
- Yoshida, H., et al. Genes. Chrom. Cancer 12 (1995) 37-44.
Analyte Specific Reagent.
Analytical and performance characteristics are not established.
Analytical and performance characteristics are not established.
