Vysis® LSI® PTEN (10q23) / CEP® 10 Dual Color Probe
Product Description
| Probe Name | Probe Location | Fluorophore |
| Vysis CEP 10 | 10p11.1-q11.1 Alpha Satellite DNA | SpectrumGreen TM |
| Vysis LSI PTEN | 10q23 | SpectrumOrange TM |
The phosphatase and tensin homolog (PTEN) on 10q23 encodes a major lipid phosphatase which negatively regulates the phosphoinositol-3-kinase/Akt pathway.
The LSI PTEN / CEP 10 Dual Color Probe is a mixture of LSI PTEN (10q23) labeled with SpectrumOrange and CEP 10 labeled with SpectrumGreen. LSI PTEN (10q23) SpectrumOrange probe is a ~370 kb probe that hybridizes to the 10q23 region on chromosome 10 and contains sequences that flank both the 5' and the 3' ends of the PTEN gene. The CEP 10 SpectrumGreen probe hybridizes to alpha satellite sequences specific to chromosome 10.
The LSI PTEN / CEP 10 Dual Color Probe is a mixture of LSI PTEN (10q23) labeled with SpectrumOrange and CEP 10 labeled with SpectrumGreen. LSI PTEN (10q23) SpectrumOrange probe is a ~370 kb probe that hybridizes to the 10q23 region on chromosome 10 and contains sequences that flank both the 5' and the 3' ends of the PTEN gene. The CEP 10 SpectrumGreen probe hybridizes to alpha satellite sequences specific to chromosome 10.
Probe Map
LSI PTEN (10q23) is a ~ 368 kb probe that hybridizes to the 10q23 region on chromosome 10 and contains sequences that flank both the 5' and 3' ends of the PTEN gene.
Results of Hybridization
In a normal cell with two intact copies of chromosome 10, two green and two orange signals will be observed. In an abnormal cell with a deletion of the PTEN (10q23) gene region, fewer than two orange signals will be observed.
Result of the hybridization of the LSI PTEN (10q23) / CEP10 Dual Color Probe as observed in normal interphase cells. (Photo courtesy of Dr. Arie Perry, Washington University.)
An abnormal cell hybridized with the LSI PTEN (10q23) / CEP10 Dual Color Probe. The cell in this image shows the one orange, two green signal pattern indicative of a PTEN (10q23) deletion. (Photo courtesy of Dr. Arie Perry, Washington University.)
References
- Goberdhan,D., et al.Human Molecular Genetics 12 (2) (2003): 239-248.
- Eng, C., et al.Human Mutation 22 (2003): 183-198.
- Sasaki, H., et al. Am. J. of Pathology 159 (1) (2001): 359-367.
Analyte Specific Reagent.
Analytical and performance characteristics are not established.
Analytical and performance characteristics are not established.
