Vysis® LSI® PML/RARA Dual Color Translocation Probe
Product Description
| Probe Name | Probe Location | Fluorophore |
| Vysis LSI RARA | 17q21 | SpectrumGreen TM |
| Vysis LSI PML | 15q22 | SpectrumOrange TM |
The LSI PML/RARA Dual Color Translocation Probe is used to detect the common t(15;17). The t(15;17) involving the PML (15q22) and RARA (17q21) genes results in the formation of the PML/RARA gene fusion product. The t(15;17) breakpoint on chromosome 15 occurs in a 13 kb region of the PML gene. There are three breakpoint cluster regions:
The LSI PML/RARA Dual Color Translocation Probe is a mixture of the LSI PML probe labeled with SpectrumOrange and the LSI RARA probe labeled with SpectrumGreen. The PML probe covers the region that begins at intron 7, a span of 5.8 kb, and extends toward the centromere of chromosome 15 for approximately 180 kb. The RARA probe begins about 500 bp 3' to exon 6 and extends approximately 400 kb toward the telomere of chromosome 17.
- bcr 3 : spans from intron 3 through the 5' end of intron 4
- bcr 2: extends from exon 5 to exon 6
- bcr 1: includes intron 6 and extends into exon 7
The LSI PML/RARA Dual Color Translocation Probe is a mixture of the LSI PML probe labeled with SpectrumOrange and the LSI RARA probe labeled with SpectrumGreen. The PML probe covers the region that begins at intron 7, a span of 5.8 kb, and extends toward the centromere of chromosome 15 for approximately 180 kb. The RARA probe begins about 500 bp 3' to exon 6 and extends approximately 400 kb toward the telomere of chromosome 17.
Probe Map
Results of Hybridization
In a normal cell, the expected pattern for a nucleus hybridized with the LSI PML/RARA probe is a two orange and two green (2O2G) signal pattern. In an abnormal cell containing a PML/RARA fusion, the one green (RARA), one orange (PML), and closely adjacent or fused green/orange (yellow) signal pattern (1O1G1F) is observed.
LSI PML/RARA Dual Color Translocation Probe hybridized to a normal nucleus, showing a two orange, two green (2O2G) signal pattern.
Analyte Specific Reagent.
Analytical and performance characteristics are not established.
Analytical and performance characteristics are not established.
