Vysis® LSI® PML/RARA Dual Color, Dual Fusion Translocation Probe
Product Description
| Probe Name | Probe Location | Fluorophore |
| Vysis LSI PML | 15q22 | SpectrumOrange TM |
| Vysis LSI RARA | 17q21.1 | SpectrumGreen TM |
The reciprocal and balanced t(15;17), involving the PML (promyelocytic leukemia) gene on chromosome 15q22 and the RARA (retinoic acid receptor alpha) gene on chromosome 17q12.1 is a characteristic molecular feature of certain types of leukemia. Two gene fusion products result from this translocation, each of which encodes a functional chimeric protein: PML/RARA and RARA/PML.
The breakpoints in the 15q22 region of chromosome 15 occur within a 13 kb region of the PML gene that contains three breakpoint cluster regions (bcr): bcr 3 extends from intron 3 through the 5' end of intron 4, bcr 2 extends from exon 5 to exon 6, and bcr 1 extends from intron 6 into exon 7. Break frequency is highest in bcr 1, followed by bcr 3 and bcr 2. The breakpoints on chromosome 17 occur within the approximately 17 kb intron 2 of the RARA gene.
The LSI PML/RARA Dual Color, Dual Fusion Translocation Probe is a mixture of two FISH DNA Probes. The first, LSI PML, is an ~500 kb unique sequence probe that hybridizes to the 15q22 region containing the PML gene and is labeled in SpectrumOrange. The second, LSI RARA, is an ~700 kb unique sequence probe that hybridizes to the 17q21.1 region containing the RARA gene and is labeled in SpectrumGreen.
The breakpoints in the 15q22 region of chromosome 15 occur within a 13 kb region of the PML gene that contains three breakpoint cluster regions (bcr): bcr 3 extends from intron 3 through the 5' end of intron 4, bcr 2 extends from exon 5 to exon 6, and bcr 1 extends from intron 6 into exon 7. Break frequency is highest in bcr 1, followed by bcr 3 and bcr 2. The breakpoints on chromosome 17 occur within the approximately 17 kb intron 2 of the RARA gene.
The LSI PML/RARA Dual Color, Dual Fusion Translocation Probe is a mixture of two FISH DNA Probes. The first, LSI PML, is an ~500 kb unique sequence probe that hybridizes to the 15q22 region containing the PML gene and is labeled in SpectrumOrange. The second, LSI RARA, is an ~700 kb unique sequence probe that hybridizes to the 17q21.1 region containing the RARA gene and is labeled in SpectrumGreen.
Probe Map
Results of Hybridization
This probe is provided for those interested in identifying the t(15;17). In a normal cell that lacks the t(15;17), a two orange and two green signal pattern will be observed reflecting the two intact copies of RARA and PML, respectively. In an abnormal cell containing the t(15;17), one orange (PML), a one green (RARA), and two fusion (PML/RARA and RARA/PML) signal pattern is observed.
Result of the hybridization of the LSI PML/RARA Dual Color, Dual Fusion Translocation Probe as observed in a normal interphase cell.
Abnormal cell hybridized with the LSI PML/RARA Dual Color, Dual Fusion Translocation Probe. The cell in this image shows the one orange (PML), one green (RARA), two fusion (PML/RARA and RARA/PML) signal pattern indicative of the t(15;17).
References
- Rowley, J.D., et al. Lancet 1 (1977): 549-550.
- Harris, N.L., et al. J. Clin. Oncol. 12 (1999): 3835-3849.
- He, L.Z., et al. Nat. Genet. 18 (1998): 126-135.
- Pandolfi, P.P. Oncogene 20. (2001): 5726-5735.
- Piazza, F., et al. Oncogene 20 (2001): 7216-7222.
- Benoit, G., et al. Oncogene 20 (2001): 7161-7177.
Analyte Specific Reagent.
Analytical and performance characteristics are not established.
Analytical and performance characteristics are not established.
