Vysis® LSI® N-MYC (2p24) SpectrumGreen/CEP® 2 SpectrumOrangeTM Probe
Product Description
| Probe Name | Probe Location | Fluorophore |
| Vysis LSI N-MYC | 2p24 | SpectrumGreen TM |
| Vysis CEP 2 | 2p11.1-q11.1 | SpectrumOrange TM |
The LSI N-MYC (2p24) probe contains unique DNA sequences specific to the N-MYC oncogene located within the 2p24 region of chromosome 2. This probe may be used to detect the N-MYC oncogene copy number.
The LSI N-MYC (2p24)/ CEP 2 (2p11.1-q11.1) Dual ColorProbe Set is a mixture of LSI N-MYC (2p24) labeled with SpectrumGreen and CEP 2 (2p11.1-q11.1) labeled with SpectrumOrange. The LSI N-MYC (2p24) SpectrumGreen probe is an approximately 200 kb probe that hybridizes to the 2p24 region on chromosome 2 and contains sequences that flank both 5' and 3' ends of the N-MYC gene. The CEP 2 (2p11.1-q11.1) SpectrumOrange probe hybridizes to alpha satellite sequences specific to chromosome 2.
The LSI N-MYC (2p24)/ CEP 2 (2p11.1-q11.1) Dual ColorProbe Set is a mixture of LSI N-MYC (2p24) labeled with SpectrumGreen and CEP 2 (2p11.1-q11.1) labeled with SpectrumOrange. The LSI N-MYC (2p24) SpectrumGreen probe is an approximately 200 kb probe that hybridizes to the 2p24 region on chromosome 2 and contains sequences that flank both 5' and 3' ends of the N-MYC gene. The CEP 2 (2p11.1-q11.1) SpectrumOrange probe hybridizes to alpha satellite sequences specific to chromosome 2.
Probe Map
LSI N-MYC (2p24) is a ~200 kb probe that hybridizes to the 2p24 region on chromosome 2 and contains sequences that flank both the 5' and 3' ends of the N-MYC gene.
Results of Hybridization
In a normal cell with two intact copies of chromosome 2, two green and two orange signals will be observed. In an abnormal cell containing amplification of the N-MYC oncogene, greater than two green signals will be observed.
An abnormal sample hybridized with the LSI N-MYC (2p24)/ CEP 2 (2p11.1-q11.1) Dual Color Probe showing a high level of amplification of the N-MYC oncogene.
References
- Brodeur, GM (2003) Nat Rev Cancer 3: 203-216.
- Brodeur, GM et al. (1984) Science 224: 1121-1124.
- Heim, S. & Mitelman, F. (1995) Cancer Cytogenetics 2nd ed.
- Maris, J., et al. (1999) J Clin Oncol 17: 2264-2279.
- Schwab, M. (2002) Ann NY Acad Sci 963: 63-73.
- Seeger, RC et al. (1985) NEJM 313:1111-1116.
Analyte Specific Reagent.
Analytical and performance characteristics are not established.
Analytical and performance characteristics are not established.
