Vysis® LSI® MLL Dual Color, Break Apart Rearrangement Probe
Product Description
| Probe Name | Probe Location | Fluorophore |
| Vysis LSI MLL | 11q23 | SpectrumOrange TM SpectrumGreen TM |
The LSI MLL Dual Color, Break Apart Rearrangement Probe is designed to detect the 11q23 rearrangement associated with various translocations involving the MLL gene. Translocations disrupting the MLL (ALL-1,HRX) gene are among the most common cytogenetic abnormalities observed in hematopoietic malignancies. Although over 30 variant translocations have been seen involving MLL translocations, the most common abnormalities are t(4;11)(q21;q23), t(9;11)(p22;q23), and t(11;19)(q23;p13).
The LSI MLL Dual Color, Break Apart Rearrangement Probe consists of a 350 kb portion centromeric of the MLL gene breakpoint cluster region (bcr) labeled in SpectrumGreen and approximately 190 kb portion largely telomeric of the bcr labeled in SpectrumOrange. In approximately 25% of 11q23 translocations, a region beginning at the MLL breakpoint and extending distally is deleted. This probe can provide a better indication of the presence of the 11q23 translocation than a single color probe design.
The LSI MLL Dual Color, Break Apart Rearrangement Probe consists of a 350 kb portion centromeric of the MLL gene breakpoint cluster region (bcr) labeled in SpectrumGreen and approximately 190 kb portion largely telomeric of the bcr labeled in SpectrumOrange. In approximately 25% of 11q23 translocations, a region beginning at the MLL breakpoint and extending distally is deleted. This probe can provide a better indication of the presence of the 11q23 translocation than a single color probe design.
Probe Map
Results of Hybridization
The signal pattern observed in a cell lacking the MLL rearrangement is expected to show a two orange/green (yellow) fusion signal pattern (2F). In a cell possessing a MLL translocation, the expected pattern is one green/orange (yellow) fusion signal, one orange signal, and one green (1O1G1F) signal. With the MLL Dual Color, Break Apart Rearrangement Probe, a large deletion occurring distally from the MLL breakpoint might weaken or totally eliminate one of the two orange signals, potentially producing a FISH pattern characteristic of concomitant translocation and deletion, i.e., one orange/green fusion and one isolated green signal.
LSI MLL Dual Color, Break Apart Rearrangement Probe hybridized to cells possessing a t(9:11)(p22;q23) and exhibiting the expected one orange, one green and one orange/green fusion signal pattern (1O1G1F).
References
- Cimino, G., et al.Haematologica 83 (1998): 350-7.
- Grimwade,D., et al. Blood 92 (1998): 2322-33.
- Marschalek, R., et al. Leuk. Lymphoma 27 (1997): 417-28.
- Rubnitz, J.E., et al. Leukemia 10 (1996): 74-82.
Analyte Specific Reagent.
Analytical and performance characteristics are not established.
Analytical and performance characteristics are not established.
