Vysis® LSI® IGH/MYC, CEP® 8 Tri-color, Dual Fusion Translocation Probe

5611_6127
LSI IGH/MYC, CEP 8 Tri-color, Dual Fusion Translocation Probe

Product Description

Probe Name Probe Location Fluorophore
Vysis LSI IGH 14q32  SpectrumGreen TM
Vysis LSI MYC 8q24  SpectrumOrange TM
Vysis CEP 8 8p11.1-q11.1  SpectrumAqua TM
The LSI IGH/MYC, CEP 8 Tri-color, Dual Fusion Translocation Probe is designed to detect the juxtaposition of immunoglobulin heavy chain (IGH) locus and MYC gene region sequences. Inclusion of the SpectrumAqua CEP 8 in this probe mixture serves as an indicator for chromosome 8 which is important when MYC amplification and loss of the der(8) chromosome occurs. The translocation t(8;14) (q24;q32) involving IGH at 14q32 and the MYC region at 8q24 is the most frequently observed MYC region translocation. The remaining cases appear to have one of the variant MYC translocations, either t(8;22)(q24;q11) or t(2;8)(p11;q24) that involve one of the two light chain immunoglobulin loci (kappa on chromosome 2 or lambda on 22). These three translocations are believed to disrupt the normal regulation of the C-MYC transcription factor by bringing the MYC gene under control of a regulatory element from one of the immunoglobulin loci.

The LSI IGH/MYC, CEP 8 Tri-color, Dual Fusion Translocation Probe is a mixture of a 1.5 Mb SpectrumGreen labeled LSI IGH probe, an 821 kb SpectrumOrange labeled LSI MYC probe and the SpectrumAqua labeled CEP 8 probe. The IGH probe contains sequences homologous to essentially the entire IGH locus as well as sequences extending about 300 kb beyond the 3' end of the IGH locus. The large MYC probe extends 385 kb upstream of MYC and 431 kb 3' beyond MYC. The CEP 8 probe targets the alpha satellite sequences on chromosome 8.

Probe Map




Results of Hybridization

In a normal cell the expected pattern for a nucleus hybridized with the LSI IGH/MYC, CEP 8 probe is the two aqua, two orange, and two green signal pattern. A cell harboring the reciprocal t(8;14) with the 8q24 breakpoint well within the MYC probe target is expected to produce a pattern of one orange, one green, two orange/green fusions, and two aqua signals (1O1G2F2A). If the cell contains a breakpoint very far 5' of MYC, a fusion on the der(8) may not be visible or may be very weak, as little or no orange probe target would remain on the der(8).

LSI IGH/MYC, CEP 8 Tri-color, Dual Fusion Translocation Probe hybridized to a normal nucleus showing the expected 202G2A signal pattern.

References

  1. Akasaka, T., et al. (2000) J Clin Oncol 18, 510-8.
  2. DeVita, V.T. (1997) Cancer: Principles & Practices of Oncology 5th ed. Philadelphia, PA, Lippincott-Raven
  3. Heim, S. & Mitelman, F. (1995) Cancer Cytogenetics 2nd ed. New York City, NY, John Wiley & Sons.
  4. Siebert, R., et al. (1998) Blood 91, 984-90.
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Order Information

Product Name Package Size Vysis Order Number Abbott Order Number
Vysis LSI IGH/MYC, CEP 8 Tri-color, Dual Fusion Translocation Probe  20 µL 32-191020 05J75-001
5J75
Analyte Specific Reagent.
Analytical and performance characteristics are not established.