Vysis® LSI® IGH/MALT1 t(14;18) (q32;q21) Dual Color, Dual Fusion Translocation Probe

5611_6522
LSI IGH/MALT1 t(14;18) (q32;q21) Dual Color, Dual Fusion Translocation Probe

Product Description

Probe Name Probe Location Fluorophore
Vysis LSI IGH 14q32  SpectrumGreen TM
Vysis LSI MALT1 18q21  SpectrumOrange TM
The LSI IGH is a 1.5 Mb probe that hybridizes to the 14q32 region on chromosome 14 and contains sequences homologous to essentially the entire IGH locus, as well as sequences extending ~ 300 kb beyond the 3' end of the IGH locus. LSI IGH is labeled in SpectrumGreen. The 670 kb SpectrumOrange labeled LSI MALT1 probe hybridizes to chromosome 18q21. It contains the entire 79 kb MALT gene and flanking sequences on either side of the gene. The probe extends from a point centromeric of the HAK gene to telomeric of the marker D18S531.

Probe Map




Results of Hybridization

In a normal cell that lacks the t(14;18)(q32;q21) translocation, a two orange, two green signal pattern will be observed reflecting two intact copies of MALT1 and IGH, respectively.

In an abnormal cell containing only the t(14;18)(q32;q21) translocation, a one orange (MALT1), one green (IGH), and two fusion (IGH/MALT1 and MALT1/IGH) signal pattern will be commonly observed. Some samples containing the t(14;18) may display signal patterns differently than one orange, one green, and two fusions.

Result of the hybridization of the LSI IGH/MALT1 t(14;18)(q32;q21) Dual Color, Dual Fusion Translocation Probe as observed in normal interphase cells.
 
An abnormal cell hybridized with the LSI IGH/MALT1 t(14;18)(q32;q21) Dual Color, Dual Fusion Translocation Probe. The cell in this image shows the one orange, one green and two fusion signal pattern indicative of the t(14;18)(q32;q21) translocation.

References

  1. Dierlamm, J., et al. Blood 93 (11) (1999): 3601-3609.
  2. Murga Penas, E.M., et al. Leukemia 17 (2003): 2225-2229.
  3. Remstein, E.D., et al. Am Jour Pathol 161 (1) (2002): 63-71.
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Order Information

Product Name Package Size Vysis Order Number Abbott Order Number
Vysis LSI IGH/MALT1 t(14;18) (q32;q21) Dual Color, Dual Fusion Translocation Probe  20 µL 32-231005 05J84-001
5J84
Analyte Specific Reagent.
Analytical and performance characteristics are not established.