Vysis® LSI® IGH/MAF Dual Color, Dual Fusion Probe

5611_6907
LSI IGH/MAF Dual Color, Dual Fusion Probe

Product Description

Probe Name Probe Location Fluorophore
Vysis LSI IGH 14q32  SpectrumGreen TM
Vysis LSI MAF 16q23  SpectrumOrange TM
Translocations of the immunoglobulin heavy chain locus (IGH) located at 14q32 are frequently observed in patients with various hematological disorders. These IGH translocations result in the upregulation of oncogenes due to the juxtaposition of IGH enhancers with these oncogenes. The t(14;16)(q32;q23) results in the dysregulation of MAF on der(14).

The LSI IGH/MAF Dual Color, Dual Fusion Translocation Probe is comprised of a mixture of an ~1.5 Mb SpectrumGreen labeled IGH probe and the two component SpectrumOrange labeled MAF probe. The LSI IGH probe contains sequences homologous to the entire IGH locus as well as sequences extending about 300 kb beyond the 3' end of the IGH locus. LSI MAF contains two SpectrumOrange labeled probes that flank the MAF locus and are separated by an ~2.2 Mb gap. A 350 kb probe located centromeric to MAF hybridizes to a region containing the RH15625 locus. A 350 kb probe located telomeric to MAF hybridizes to a region containing both the RH69965 and SHGC-4903 loci.

Probe Map




Results of Hybridization

In a normal cell that lacks the t(14;16), a two green and two orange signal pattern will be observed reflecting the two intact copies of IGH and the MAF region respectively. Due to the presence of the ~2.2 Mb gap between the two SpectrumOrange labeled MAF probes, signal splitting of the orange probe may be observed in both normal and abnormal cells. In an abnormal cell containing the t(14;16), one green (IGH), one orange (MAF) and two fusion signal pattern (der (14) and der (16)) may be observed. Some samples containing the t(14;16) may display signal patterns different than one orange, one green and two fusions.

A normal interphase cell hybridized with the LSI IGH/MAF Dual Color, Dual Fusion Translocation Probe. The cell shows the expected two green (IGH), two orange (MAF) signal pattern.
An abnormal interphase cell hybridized with the LSI IGH/MAF Dual Color, Dual Fusion Translocation Probe. The cell in this image shows the one green (IGH), one orange (MAF), two fusion (der (14) and der (16)) signal pattern indicative of a t(14;16).

References

  1. Chesi M et al. Blood 91 (12) (1998): 4457-4463.
  2. Fonseca R et al. Blood 101 (11) (2003): 4569-4575.
  3. Fonseca R et al. Cancer Research 64 (2004): 1546-1558.
  4. Hurt E et al. Cancer Cell (5) (2004): pp.191-199.
Back To Top

Order Information

Product Name Package Size Vysis Order Number Abbott Order Number
Vysis LSI IGH/MAF Dual Color, Dual Fusion Probe  20 µL 32-231014 05J84-004
5J84
Analyte Specific Reagent.
Analytical and performance characteristics are not established.