Vysis® LSI® IGH/FGFR3 Dual Color, Dual Fusion Translocation Probe
Product Description
| Probe Name | Probe Location | Fluorophore |
| Vysis LSI IGH | 14q32 | SpectrumGreen TM |
| Vysis LSI FGFR3 | 4p16 | SpectrumOrange TM |
Translocations of the immunoglobulin heavy chain locus (IGH) located at 14q32 are frequently observed in patients with various hematological disorders. These IGH translocations result in the upregulation of oncogenes due to the juxtaposition of IGH enhancers with these oncogenes.
The LSI IGH/FGFR3 Dual Color, Dual Fusion Translocation Probe is comprised of a mixture of a 1.5 Mb SpectrumGreen labeled IGH probe and an ~900 Kb SpectrumOrange labeled FGFR3 probe.
The IGH probe contains sequences homologous to the entire IGH locus, as well as sequences extending about 300 kb beyond the 3' end of the IGH locus.
The FGFR3 probe contains sequences that extend from a point 460 Kb telomeric to FGFR3, through the FGFR3 and WHSC1 (Wolf-Hirschhorn Syndrome Candidate 1) genes and ends proximally at a locus ~360 Kb centromeric to FGFR3.
The LSI IGH/FGFR3 Dual Color, Dual Fusion Translocation Probe is comprised of a mixture of a 1.5 Mb SpectrumGreen labeled IGH probe and an ~900 Kb SpectrumOrange labeled FGFR3 probe.
The IGH probe contains sequences homologous to the entire IGH locus, as well as sequences extending about 300 kb beyond the 3' end of the IGH locus.
The FGFR3 probe contains sequences that extend from a point 460 Kb telomeric to FGFR3, through the FGFR3 and WHSC1 (Wolf-Hirschhorn Syndrome Candidate 1) genes and ends proximally at a locus ~360 Kb centromeric to FGFR3.
Probe Map
Results of Hybridization
In a normal cell that lacks the t(4;14), a two orange and two green signal pattern will be observed reflecting the two intact copies of the FGFR3 and IGH regions respectively. In an abnormal cell containing the t(4;14), one orange (FGFR3), one green (IGH), and two fusion signal pattern (der (4) and der (14)) may be observed. Some samples containing the
t(4;14) may display signal patterns differently than one orange, one green, and two fusions.
A normal interphase cell hybridized with the LSI IGH/FGFR3 Dual Color, Dual Fusion Translocation Probe. The cell shows the expected two orange (FGFR3), two green (IGH) signal pattern.
An abnormal interphase cell hybridized with the LSI IGH/FGFR3 Dual Color, Dual Fusion Translocation Probe. The cell in this image shows the one orange (FGFR3), one green (IGH), two fusion (der (4) and der (14)) signal pattern indicative of a t(4;14).
References
- Fonseca, R., et al. Blood 101 (11) 2003: 4569-4575.
- Keats, J., et al. Blood 101 (4) 2003: 1520-1529.
- Chesi, M., et al. Blood 92 (1) 1998: 3025-3034.
Analyte Specific Reagent.
Analytical and performance characteristics are not established.
Analytical and performance characteristics are not established.
