Vysis® LSI® IGH/CCND1 Dual Color, Dual Fusion Translocation Probe

5611_6522
LSI IGH/CCND1 Dual Color, Dual Fusion Translocation Probe

Product Description

Probe Name Probe Location Fluorophore
Vysis LSI CCND1 11q13  SpectrumOrange TM
Vysis LSI IGH 14q32  SpectrumGreen TM
The LSI IGH/CCND1 Dual Color, Dual Fusion Translocation Probe is designed to detect the juxtaposition of the immunoglobulin heavy chain (IGH) locus and the Cyclin D1 gene (CCND1).

The LSI IGH/CCND1 Dual Color, Dual Fusion Translocation Probe is a mixture of the LSI IGH probe labeled with SpectrumGreen and the LSI CCND1 probe labeled with SpectrumOrange. The LSI IGH probe set contains two SpectrumGreen labeled probes that hybridize to sequences on either side of the IGH J region breakpoint associated with the t(11;14).

The LSI CCND1 probe has a hybridization target length of approximately 350 kb and spans the common breakpoint region (MTC major translocation cluster) that lies centromeric to LSI CCND1.

Probe Map


 


Results of Hybridization

LSI IGH/CCND1 hybridized to a cell containing t(11;14) with breakpoints at the MTC on 11q13 and at the IGH J region on 14q32 is expected to result in a signal pattern of two orange/green (yellow) fusions, one on each of the abnormal chromosomes 11 and 14 and single orange and green signals from the normal chromosomes.

Due to the gap between the two probes in the IGH probe set, the normal IGH loci may sometimes appear as two slightly separated green signals. This gap may also cause a slight separation of the orange and green signals on the der(11) chromosome, in some instances.  Analysis of t(11;14) samples suggests that due to variation in breakpoint location on 11q13 loss of V segments within the LSI IGH probe target, some samples containing t(11;14) might display signal patterns different than 1O1G2F.

LSI IGH/CCND1 Dual Color, Dual Fusion Translocation Probe hybridized to an abnormal nucleus showing the common 1O1G2F signal pattern.

References

  1. de Boer, C.J., et al. Ann Oncol 8 (1997): 109-17.
  2. Kluin, P.H., Schuuring, E. Cancer Surv 30 (1997): 3-20.
  3. Li, J.Y., et al. Am J Pathol 154 (1999): 1449-52.
  4. Vaandrager, J.W., et al. Blood 88 (1997): 1177-82.
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Order Information

Product Name Package Size Vysis Order Number Abbott Order Number
Vysis LSI IGH/CCND1 Dual Color, Dual Fusion Translocation Probe  20 µL 32-191017 05J69-001
5J69
Analyte Specific Reagent.
Analytical and performance characteristics are not established.