Vysis® LSI® IGH/BCL2 Dual Color, Dual Fusion Translocation Probe

5611_6522
LSI IGH/BCL2 Dual Color, Dual Fusion Translocation Probe

Product Description

Probe Name Probe Location Fluorophore
Vysis LSI IGH 14q32  SpectrumGreen TM
Vysis LSI BCL2 18q21  SpectrumOrange TM
The LSI IGH/BCL2 Dual Color, Dual Fusion Translocation Probe is designed to detect the juxtaposition of immunoglobulin heavy chain (IGH) locus and BCL gene sequences. The translocation involving IGH at 14q32 and BCL2 at 18q21, t(14;18)(q32;q21) is common. Relocation of an IGH transcriptional enhancer next to the BCL2 gene as a result of the t(14;18) translocation is thought to cause constitutive over-expression of the anti-apoptotic BCL2 protein. The breakpoints at 14q32 occur at the IGH J segments and about 75% of the breaks at 18q21 occur in either the 2.8 kb major breakpoint region (MBR) 3' of BCL2 exon 3 or in the minor cluster region (MCR) located about 30 kb 3' to the MBR. The remaining BCL2 breakpoints are thought to lie between the MBR and MCR regions, or 5' of the BCL2 gene.

The LSI IGH/BCL2 Dual Color, Dual Fusion Translocation Probe is a mixture of the LSI IGH probe labeled with SpectrumGreen spanning approximately 1.5 Mb and containing sequences homologous to essentially the entire IGH locus, as well as sequences extending about 300 kb beyond the 3' end of the IGH locus. The LSI BCL2 probe labeled with SpectrumOrange covers an approximate 750 kb region, including the entire BCL2 gene with additional sequences extending approximately 250 kb both distal and proximal to the gene.

Probe Map




Results of Hybridization

The expected pattern in a normal nucleus hybridized with the LSI IGH/BCL2 probe is the two orange, two green signal pattern (2O2G). In a nucleus harboring a t(14;18), the most common pattern is one orange signal, one green signal (representing the normal homolog) and two orange/green (yellow) fusion signals representing the two derivative chromosomes resulting from the reciprocal translocation (1O1G2F pattern). Patterns other than 1O1G2F may be observed in some abnormal cells including instances of nuclei containing more than two fusion signals.

LSI IGH/BCL2 Dual Color, Dual Fusion Translocation Probe hybridized to a nucleus containing the t(14;18) (q32;q21) showing the 1O1G2F signal pattern.

References

  1. Heim, S., et al. Cancer Cytogenetics 2nd ed. (1995): 377.
  2. Offitt, K. Leuk Lymphoma 7 (1992): 275-82.
  3. Vaandrager, J.W., et al. Genes Chrom Cancer 25 (2000): 85-94.
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Order Information

Product Name Package Size Vysis Order Number Abbott Order Number
Vysis LSI IGH/BCL2 Dual Color, Dual Fusion Translocation Probe  20 µL 32-191018 05J71-001
5J71
Analyte Specific Reagent.
Analytical and performance characteristics are not established.