Vysis® LSI® ETV6 (TEL) (12p13) Dual Color, Break Apart Rearrangement Probe
Product Description
| Probe Name | Probe Location | Fluorophore |
| Vysis LSI ETV6 (TEL) | 12p13 | SpectrumOrange TM SpectrumGreen TM |
Hybridization with the Vysis LSI ETV6 Dual Color, Break Apart Rearrangement Probe will identify rearrangements at the ETV6 gene region, regardless of what other chromosomal regions are involved. For example, this will allow detection of a chromosomal break caused by a translocation in the ETV6 gene region even when the translocation partner is unknown.
The Vysis LSI ETV6 (TEL) (12p13) Dual Color, Break Apart Rearrangement Probe is a mixture of two probes. The first probe, a 630 kb probe labeled in SpectrumGreen begins about 6 kb proximal to the ETV6 (TEL) gene and extends to toward the centromere. The second probe, labeled in SpectrumOrange, begins within ETV6 intron 2 and extends toward the 12p telomere for approximately 490 kb. There is a gap between the two probes of about 140 kb.
The Vysis LSI ETV6 (TEL) (12p13) Dual Color, Break Apart Rearrangement Probe is a mixture of two probes. The first probe, a 630 kb probe labeled in SpectrumGreen begins about 6 kb proximal to the ETV6 (TEL) gene and extends to toward the centromere. The second probe, labeled in SpectrumOrange, begins within ETV6 intron 2 and extends toward the 12p telomere for approximately 490 kb. There is a gap between the two probes of about 140 kb.
Probe Map
Results of Hybridization
Hybridization of this probe to interphase nuclei of normal cells is expected to produce two pair of overlapping, or nearly overlapping, orange and green (yellow fusion) signals. The anticipated signal pattern in abnormal cells having a chromosomal breakpoint within the gap between the two probe targets on one chromosome 12 is one orange, one green, and one fusion signal. Other patterns may be observed if additional genetic alterations are present.
Normal cell hybridization using the LSI ETV6 (TEL) (12p13) Dual Color, Break Apart Rearrangement Probe.
Abnormal cell hybridization using the LSI ETV6 (TEL) (12p13) Dual Color, Break Apart Rearrangement Probe.
References
- Golub T., et al. (1995) Proc. Natl. Acad. Sci. USA 92: 4917-4921.
Analyte Specific Reagent.
Analytical and performance characteristics are not established.
Analytical and performance characteristics are not established.
