Vysis® LSI® EGFR SpectrumOrangeTM / CEP® 7 SpectrumGreenTM Probe
Product Description
| Probe Name | Probe Location | Fluorophore |
| Vysis LSI EGFR | 7p12 | SpectrumOrange TM |
| Vysis CEP 7 | 7p11.1-q11.1 Alpha Satellite DNA | SpectrumGreen TM |
The LSI EGFR/CEP 7 Dual Color Probe is designed to detect and quantify copy number of the epidermal growth factor receptor gene (EGFR) located at 7p12. The EGF receptor, alternately known as HER-1/erb-B1, is a member of the EGFR family. Also included in this family are HER-2/erb-B2,HER-3/erb-B3, and HER-4/erb-B4. The EGFR gene codes for a transmembrane glycoprotein with tyrosine kinase activity,which presumably plays a key role in controlling cell proliferation.
The LSI EGFR Probe is labeled in SpectrumOrange and covers an approximately 300 kb region that contains the entire EGFR gene. The CEP 7 probe, labeled in SpectrumGreen, hybridizes to the alpha satellite DNA located at the centromere of chromosome 7 (7p11.1-q11.1). This probe set is premixed in Hybridization Buffer.
The LSI EGFR Probe is labeled in SpectrumOrange and covers an approximately 300 kb region that contains the entire EGFR gene. The CEP 7 probe, labeled in SpectrumGreen, hybridizes to the alpha satellite DNA located at the centromere of chromosome 7 (7p11.1-q11.1). This probe set is premixed in Hybridization Buffer.
Probe Map
Results of Hybridization
In a cell with normal copy number of the EGFR gene and chromosome 7, two orange signals (EGFR), and two green signals (chromosome 7) will be observed.Abnormal copy number of the EGFR gene is indicated by three or more copies of the orange probe signal. Simultaneously, the copy number of chromosome 7 can be quantified by enumeration of the green signals observed within the same cell. Therefore, enumeration of both the orange EGFR and green CEP 7 signals provide a mechanism for determining EGFR copy number relative to total chromosome 7 copy number.
LSI EGFR/CEP 7 Dual Color Probe hybridized to a nucleus indicating greater than 24 copies (orange signals) of the EGFR gene and four copies of chromosome 7 (green signals).
References
- Bredel, M., et al. (1999) Clin Cancer Res 5, 1786-92.
- Harris, A., et al. (1989) J Steroid Biochem 34, 123-31.
- Kitagawa, Y., et al. (1996) Clin Cancer Res 2, 909-14.
- Neal, D.E., et al. (1990) Cancer 65, 1619-25.
- Osaki, A., et al. (1992) Am J of Surg 164, 323-6.
- Pavelic, K., et al. (1993) Anticancer Res 13, 1133-7.
- Sauter, G., et al. (1996) Am J Pathol 148, 1047-53.
- Torregrosa, D., et al. (1997) Clin Chim Acta 262, 99-119.
Analyte Specific Reagent.
Analytical and performance characteristics are not established.
Analytical and performance characteristics are not established.
