Vysis® LSI® Cyclin D1 (11q13) SpectrumOrangeTM / CEP® 11 SpectrumGreenTM Probe

20358_5367
LSI Cyclin D1 (11q13) SpectrumOrange/CEP 11 SpectrumGreen Probe

Product Description

Probe Name Probe Location Fluorophore
Vysis LSI Cyclin D1 11q13  SpectrumOrange TM
Vysis CEP 11 11p11.11-q11 Alpha Satellite DNA SpectrumGreen TM
The Vysis LSI Cyclin D1 (11q13) SpectrumOrange/ CEP 11 SpectrumGreen Probe is a mixture of two probes, The CCND1 probe is approximately 300 kb , contains the CCND1 gene, and is labeled in SpectrumOrange. The second probe is specific to the D11Z1 alpha satellite centromeric repeat of chromosome 11 and is labeled in SpectrumGreen.

Probe Map



Results of Hybridization

Hybridization of this probe to interphase nuclei of normal cells is expected to produce two orange and two green signals. The anticipated signal pattern in abnormal cells having a gain of copy number of the CCND1 target without a gain of the CEP 11 target is two green and multiple orange signals. Other patterns may be observed if additional genetic alterations are present.

LSI Cyclin D1 SpectrumOrange and CEP 11 SpectrumGreen hybridized to abnormal tissue.

References

  1. Heim, F., et al. Cancer Cytogenetics 2nd ed. (1995): 377.
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Order Information

Product Name Package Size Vysis Order Number Abbott Order Number
Vysis LSI Cyclin D1 (11q13) SpectrumOrange/CEP 11 SpectrumGreen Probe  20 µL 32-191039 05J41-001
5J41
Analyte Specific Reagent.
Analytical and performance characteristics are not established.