Vysis® LSI® CCND1 (11q13) Dual Color, Break Apart Rearrangement Probe
Product Description
| Probe Name | Probe Location | Fluorophore |
| Vysis LSI CCND1 | 11q13 | SpectrumOrange TM SpectrumGreen TM |
Hybridization with the Vysis LSI CCND1 (11q13) Dual Color, Break Apart Rearrangement Probe will identify rearrangements at the CCND1 (Cyclin D1) gene region, also known the bcl-1 gene region, on chromosome 11q13, regardless of what other chromosomal regions are involved. For example, this will allow detection of a chromosomal break caused by a translocation in the CCND1 gene region even when the translocation partner is unknown.
The Vysis LSI CCND1(11q13) Dual Color, Break Apart Rearrangement Probe is a mixture of two probes, The first probe, labeled in SpectrumGreen, is approximately 700 kb in length and lies centromeric to CCND1 and MYEOV. The second probe, labeled in SpectrumOrange, begins 2.6 kb centromeric of CCND1 and extends for about 530 kb toward the 11q telomere. There is a gap of about 420 kb between the two probe components.
The Vysis LSI CCND1(11q13) Dual Color, Break Apart Rearrangement Probe is a mixture of two probes, The first probe, labeled in SpectrumGreen, is approximately 700 kb in length and lies centromeric to CCND1 and MYEOV. The second probe, labeled in SpectrumOrange, begins 2.6 kb centromeric of CCND1 and extends for about 530 kb toward the 11q telomere. There is a gap of about 420 kb between the two probe components.
Probe Map
The Vysis LSI CCND1 (11q13) Dual Color, Break Apart Rearrangement Probe is a mixture of two probes. The first probe, a 700 kb probe labeled in SpectrumGreen, lies proximal to the CCND1 gene. The second probe, labeled in SpectrumOrange, extends distally from the CCND1 gene and is 530 kb in length.
Results of Hybridization
Hybridization of this probe to interphase nuclei of normal cells is expected to produce two pair of overlapping, or nearly overlapping, orange and green (yellow fusion) signals. The anticipated signal pattern in abnormal cells having a chromosomal breakpoint within the gap between the two probe targets on one chromosome 11 is one orange, one green, and one fusion signal. Other patterns may be observed if additional genetic alterations are present.
Normal cell hybridization using the LSI CCND1 (11q13) Dual Color, Break Apart Rearrangement Probe.
Abnormal cell hybridization using the LSI CCND1 (11q13) Dual Color, Break Apart Rearrangement Probe.
References
- Degan M., et al. (2002) J Pathol 197: 256-263.
- Galiegue-Zouitina S., et al. (1994) Chromosomes Cancer 11: 246-255.
- Williams ME., et al. (1991) Blood 78: 493-498.
Analyte Specific Reagent.
Analytical and performance characteristics are not established.
Analytical and performance characteristics are not established.
