Vysis® LSI® BCR/ABL ES Dual Color Translocation Probe
Product Description
| Probe Name | Probe Location | Fluorophore |
| Vysis LSI ASS-ABL | 9q34 | SpectrumOrange TM |
| Vysis LSI BCR | 22q11.2 | SpectrumGreen TM |
The BCR/ABL ES Dual Color Translocation Probe is a mixture of the LSI ABL probe labeled with SpectrumOrange and the LSI BCR probe labeled with SpectrumGreen. The spanning ABL probe is approximately 650 kb extending from an area centromeric of the ASS gene to well telomeric of the last ABL exon. The SpectrumGreen BCR probe is approximately 300 kb beginning between BCR exons 13 and 14 (M-bcr exons 2 and 3) and extending well beyond the m-bcr region.
Probe Map
Results of Hybridization
A nucleus lacking the t(9;22) will exhibit a two orange, two green (2O2G) signal pattern. In a nucleus possessing the t(9;22) involving the M-bcr, one green (native BCR), one large orange (native ABL), one smaller orange (ES), and one fused orange/green signal (5' BCR/3' ABL), (2O1G1F) will be observed. Minor breakpoint (m-bcr) signal patterns may appear as one orange, one green, and two fusion signals. In some cells a deletion may occur 5' of the ABL breakpoint that may reduce the ES pattern to a single fusion pattern.
LSI BCR/ABL ES Dual Color Translocation Probe hybridized to a nucleus containing the t(9;22) showing one green (native BCR), one large orange (native ABL), one smaller orange (ES) and one fused orange/green (20IGIF) signal pattern.
References
- P.B. Sinclair, A.R. Green, C. Grace and E.P. Nacheva et. al., 1997. Blood 90(4)
Analyte Specific Reagent.
Analytical and performance characteristics are not established. CE Marked Not available in United States
Analytical and performance characteristics are not established. CE Marked Not available in United States
