Vysis^® LSI^® BCR/ABL Dual Color, Single Fusion Translocation Probe

Product Description

Probe Name	Probe Location	Fluorophore
Vysis LSI ABL	9q34	SpectrumOrange ^TM
Vysis LSI BCR	22q11.2	SpectrumGreen ^TM

The LSI BCR/ABL Dual Color, Single Fusion Translocation Probe is a mixture of the LSI ABL probe labeled with SpectrumOrange and the LSI BCR probe labeled with SpectrumGreen. The ABL probe begins between exons 4 and 5 and continues for about 300 kb toward the telomere of chromosome 9. The LSI BCR probe begins between BCR exons 13 and 14 (M-bcr exons 2 and 3) and extends toward the centromere on chromosome 22 for approximately 300 kb, extending well beyond the m-bcr region.

Probe Map

Results of Hybridization

A nucleus lacking the t(9;22) will exhibit the two orange, two green (2O2G) signal pattern. In a cell harboring the t(9;22), one orange, one green, and one orange/green (yellow) fusion signal pattern (1O1G1F) will be observed. This simple probe design detects the 5' BCR/3' ABL gene fusion and is useful for detecting samples with a high percentage of cells possessing this translocation.

LSI BCR/ABL Dual Color, Single Fusion Translocation Probe hybridized to a nucleus containing the t(9;22). One orange, one green and one fusion (IOIGIF) signal pattern is observed.

References

Dewald, G.W., et al. Blood 91 (1998): 3357-65.
Dewald, G.W., et al. Leuk Lymphoma 34 (1999): 481-91.
Mahon, F.X. Blood 96 (2000): 1070-9.
Sinclair, P., et al. Blood 95 (2000): 738-43.
Sinclair, P., et al. (2000) Blood 95, 738-43.

Order Information

Product Name	Status	Package Size	Vysis Order Number	Abbott Order Number
Vysis LSI BCR/ABL Dual Color, Single Fusion Translocation Probe	ASR	20 µL	32-190022	05J77-001 5J77
Vysis LSI BCR/ABL Dual Color, Single Fusion Translocation Probe		20 µL	32-160022	08L56-050 8L56

Analyte Specific Reagent.
Analytical and performance characteristics are not established.

CE Marked Not available in United States