Vysis® LSI® BCR/ABL Dual Color, Dual Fusion Translocation Probe
Product Description
| Probe Name | Probe Location | Fluorophore |
| Vysis LSI ASS-ABL | 9q34 | SpectrumOrange TM |
| Vysis LSI BCR | 22q11.2 | SpectrumGreen TM |
The LSI BCR/ABL Dual Color, Dual Fusion Translocation Probe is a mixture of the LSI BCR probe labeled with SpectrumGreen and the LSI ABL probe labeled with SpectrumOrange. The spanning ABL probe has a genomic target of approximately 650 kb extending from an area centromeric of the argininosuccinate synthetase gene (ASS) to well telomeric of the last ABL exon. The BCR probe target spans a genomic distance of about 1.5 Mb. The BCR probe begins within the variable segments of the immunoglobulin lambda light chain locus (IGLV), extends along chromosome 22 through the BCR gene, and ends at a point approximately 900 kb telomeric of BCR. A region of about 300 kb containing low-copy number repeats has been eliminated from the probe which introduces a gap in the coverage of the probe target. Both probes span their respective breakpoints.
Probe Map
Results of Hybridization
A nucleus lacking the t(9;22) translocation will exhibit the two orange, two green (2O2G) signal pattern. In a nucleus containing a simple balanced t(9;22), one orange and one green signal from the normal 9 and 22 chromosomes and two orange/green (yellow) fusion signals, one each from the derivative 9 and 22 chromosomes, will be observed (1O1G2F). In some instances, deletions may occur 3' of the BCR breakpoint and/or 5' of the ABL breakpoint resulting in either an ES (extra orange or green) signal pattern or a single fusion pattern.
LSI BCR/ABL Dual Color, Dual Fusion Translocation Probe hybridized to a nucleus containing a simple balanced t(9;22). One orange, one green and two orange/green fusion signals are observed (1O1G2F).
Order Information
| Product Name | Status | Package Size | Vysis Order Number | Abbott Order Number |
| Vysis LSI BCR/ABL Dual Color, Dual Fusion Translocation Probe | ASR | 20 µL | 32-191032 | 05J82-001 5J82
|
| Vysis LSI BCR/ABL Dual Color, Dual Fusion Translocation Probe |  |
20 µL | 32-162032 | 08L10-001 8L10
|
| Vysis LSI BCR/ABL Dual Color, Dual Fusion Translocation Probe | ASR | 50 µL | 35-191032 | 05J82-010 5J82
|
| Vysis LSI BCR/ABL Dual Color, Dual Fusion Translocation Probe | |
50 µL | 35-162032 | 08L10-002 8L10
|
Analyte Specific Reagent.
Analytical and performance characteristics are not established. CE Marked Not available in United States
Analytical and performance characteristics are not established. CE Marked Not available in United States
