Vysis® LSI® BCL6 Dual Color, Break Apart Rearrangement Probe

5611_6522
LSI BCL6 Dual Color, Break Apart Rearrangement Probe

Product Description

Probe Name Probe Location Fluorophore
Vysis LSI BCL6 3q27  SpectrumOrange TM
SpectrumGreen TM
The LSI BCL6 Dual Color, Break Apart Rearrangement Probe provides a simple and rapid method of detecting chromosome breaks associated with a number of different translocations that involve the BCL6 gene located on chromosome 3.

The LSI BCL6 Dual Color, Break Apart Rearrangement Probe is a mixture of an approximately 300 kb labeled SpectrumOrange 5' LSI BCL6 probe and a 600 kb labeled SpectrumGreen 3' LSI BCL6 probe. These two probes are separated by a 42 kb gap that contains the entire BCL6 gene, including the BCL6 breakpoint region.

Probe Map



Results of Hybridization

In a normal cell hybridized with the BCL6 probe, the expected signal pattern is two orange/green (2F) fusion signals. In a cell with a translocation breakpoint within the gap region between the 5' and 3' BCL6 probe hybridization targets one orange, one green and one fusion pattern (1O1G1F) is observed. Some normal nuclei may appear to have a slight separation of the orange and green signals due to a gap between the hybridization targets of the two probes.

LSI BCL6 Dual Color, Break Apart Rearrangement Probe hybridized to a normal nucleus showing the expected two orange/green fusion (2F) signal pattern.

References

  1. Martinez-Climent, J., et al. Blood 96 (2000): Abstract #361 84a.
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Order Information

Product Name Package Size Vysis Order Number Abbott Order Number
Vysis LSI BCL6 Dual Color, Break Apart Rearrangement Probe  20 µL 32-191016 05J68-001
5J68
Analyte Specific Reagent.
Analytical and performance characteristics are not established.