Vysis® LSI® AML1/ETO Dual Color, Dual Fusion Translocation Probe
Product Description
| Probe Name | Probe Location | Fluorophore |
| Vysis LSI AML1 | 21q22 | SpectrumGreen TM |
| Vysis LSI ETO | 8q22 | SpectrumOrange TM |
The LSI AML1/ETO Dual Color, Dual Fusion Translocation Probe is designed to detect the juxtaposition of the AML1 gene locus on chromosome 21q22 with the ETO gene locus on chromosome 8q22. The 8q22 breakpoint clusters are within the putative zinc finger DNA binding gene ETO (CBFA2T1). The breakpoints in 21q22 are clustered within the AML1 (RUNX1) gene. The 8;21 translocation event produces a fusion of the two genes on the derivative 8 chromosome that results in the novel chimeric gene, AML1/ETO. Variant translocations involving chromosomes 8 and 21 also exist and can be missed in an unusual or complex karyotype.
The LSI AML1/ETO Dual Color, Dual Fusion Translocation Probe is a mixture of a SpectrumGreen labeled LSI AML1 (21q22) probe and a SpectrumOrange labeled LSI ETO (8q22) probe. The approximately 1.3 Mb LSI AML1 probe hybridizes to the 21q22 band containing the AML1 gene. The LSI ETO is a 480 kb probe that hybridizes to the 8q22 band containing the ETO gene.
The LSI AML1/ETO Dual Color, Dual Fusion Translocation Probe is a mixture of a SpectrumGreen labeled LSI AML1 (21q22) probe and a SpectrumOrange labeled LSI ETO (8q22) probe. The approximately 1.3 Mb LSI AML1 probe hybridizes to the 21q22 band containing the AML1 gene. The LSI ETO is a 480 kb probe that hybridizes to the 8q22 band containing the ETO gene.
Probe Map
Results of Hybridization
In a normal cell without the AML1/ETO fusion gene, two orange signals representing normal copies of ETO and two green signals representing normal copies of AML1 are observed. In a cell containing the AML1/ETO fusion gene, one orange (ETO), one green (AML1), and two orange/green (yellow) fusion signals are observed. The fusion signals represent the juxtaposition of the translocated portions of the two gene regions on the der(8) and the der(21). Variant AML1/ETO signal patterns other than the most commonly observed one orange, one green, and two fusions (1O1G2F), may also occur.
LSI AML1/ETO Dual Color, Dual Fusion Translocation Probe hybridized to an abnormal nucleus showing a one orange, one green and two fusion (1O1G2F) signal pattern.
References
- Byrd, J., et al. (1999) J Clin Oncol 17, 3767-75.
- Harrison, C.J., et al. (1999) Cancer Genet Cytogent 112, 15-20.
- Mitelman, F., et al. (1992) Genes Chrom Cancer 5, 57-66.
- Gordon, C., et al. Leukemia 16 (2002): 1752-1754.
Analyte Specific Reagent.
Analytical and performance characteristics are not established.
Analytical and performance characteristics are not established.
