Vysis® LSI® 1p36 / LSI 1q25 and LSI 19q13/19p13 Dual-Color Probe
Product Description
| Probe Name | Probe Location | Fluorophore |
| Vysis LSI 1p36 | 1p36 | SpectrumOrange TM |
| Vysis LSI 1q25 | 1q25 | SpectrumGreen TM |
| Vysis LSI 19p13 | 19p13 | SpectrumGreen TM |
| Vysis LSI 19q13 | 19q13 | SpectrumOrange TM |
The LSI 1p36 / LSI 1q25 Dual Color Probes are provided in one vial as a mixture of a ~ 620 kb SpectrumGreen labeled 1q25 probe and a 400 kb SpectrumOrange labeled 1p36 probe. The LSI 1p36 probe contains sequences that extend from near SHGC57243 locus, through the TP73 and EGFL3 genes, and ends proximally at a point telomeric to the EGFL3 locus. The LSI 1q25 probe contains sequences that extend from a point telomeric to the ABL2 locus, through the ABL2 and ANGPTL1 genes, and ends proximally near the SHGC-1322 locus. The LSI 19p13 / LSI 19q13 Dual Color Probes are provided in one vial as a mixture of a ~500 kb SpectrumGreen labeled 19p13 probe and a ~ 400 kb SpectrumOrange labeled 19q13 probe. The LSI 19p13 probe contains sequences that extend from a point centromeric to the MAN2B1 locus, through MAN2B1, ZNF443 and ZNF44 genes, and ends proximally at a point telomeric to the ZNF44 locus. The LSI 19q13 probe contains sequences that extend from a point telomeric to the CRX locus, through the CRX, GLTSCR2 and GLTSCR1 genes, and ends proximally at a point centromeric to the GLTSCR1 locus. Both probe mixtures are premixed in Hybridization Buffer.
Probe Map
Results of Hybridization
As indicated above, this product contains two vials containing dual color probes. A description of the hybridization results expected with each probe set follows below:Vial 1
This probe allows status assessment of the following two chromosome regions: 1p36 and 1q25. In a normal cell hybridized with the LSI 1p36 and LSI 1q25, two orange and two green signals will be observed indicative of two intact copies of chromosome 1. In an abnormal cell with a deletion in the 1p36 region fewer than two orange signals will be observed.
Vial 2
This probe allows status assessment of the following two chromosome regions: 19q13 and 19p13. In a normal cell with two intact copies of chromosome 19, two orange and two green signals will be observed. In an abnormal cell with a deletion in the 19q13 region fewer than two orange signals will be observed.
Result of the hybridization of the LSI 1p36 / LSI 1q25 Dual-Color Probe vial 1 as observed in normal interphase cells.
An abnormal cell hybridized with the LSI 1p36 / LSI 1q25 Dual-Color Probe vial 1. The cell in this image shows the one orange, two green signal pattern indicative of the 1p36 deletion.
Result of the hybridization of the LSI 19p13 / LSI 19q13 Dual-Color Probe vial 2 as observed in normal interphase cells.
An abnormal cell hybridized with the LSI 19p13 / LSI 19q13 Dual-Color Probe vial 2. The cell in this image shows the one orange, two green signal pattern indicative of the 19q13 deletion.
References
- Cairncross J et al. (1998) J. of the National Cancer Institute 90(19): 1473-1479.
- Smith J et al. (1999) Oncogene 18: 4144-4152
- Ino Y et al. (2001) Clinical Cancer Research 7: 839-845.
- Perry A et al. (2003) Frontiers in Bioscience 8: 1-9.
- Buckner J et al. (2003) J of Clinical Oncology 21 (2): 251-255).
Analyte Specific Reagent.
Analytical and performance characteristics are not established.
Analytical and performance characteristics are not established.
