HYBriteTM Recommended Settings
| Probe/Specimen | Melt | Hyb |
| Lymphocytes | ||
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| WCP® ® | 70°C x 3 min | 37°C O/N* |
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| CEP® ® | 73°C x 1 min | 42°C x 30 min-O/N |
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| LSI® ® | 73°C x 2 min | 37°C O/N |
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| Bone Marrow | 73°C x 2 min | 37°C x 1 hour-O/N CEP 37°C x O/N LSI® |
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| Uncultured Amniocyte | 73°C x 2 min | 37°C x 4 hrs-O/N |
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| Cultured Amniocyte | 73°C x 2 min | 37°C x 4 hrs-O/N |
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| Paraffin-Embedded | 73°C x 6 min | 37°C x 16 hr(minimum) |
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| Tissue Blastomere | 73°C x 5 min | 37°C x 4 hrs |
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| (See MultiVysion™ PGT HYBriteTM protocol for specific information)** | ||
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| Polar Body | 69°C x 8 min | 37°C x 4 hrs |
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| Uroepithelial | 73°C x 2 min | 39°C x 4 hrs-O/N |
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| Probe/Specimen | Melt | Hyb |
| Lymphocytes | ||
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| WCP® | 70°C x 3 min | 37°C O/N* |
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| CEP® | 73°C x 1 min | 42°C x 30 min-O/N |
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| LSI® | 73°C x 2 min | 37°C O/N |
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| Bone Marrow | 73°C x 2 min | 37°C x 1 hour-O/N CEP 37°C x O/N LSI® |
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| Uncultured Amniocyte | 73°C x 2 min | 37°C x 4 hrs-O/N |
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| Cultured Amniocyte | 73°C x 2 min | 37°C x 4 hrs-O/N |
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| Paraffin-Embedded | 73°C x 6 min | 37°C x 16 hr(minimum) |
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| Tissue Blastomere | 73°C x 5 min | 37°C x 4 hrs |
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| (See MultiVysion™ PGT HYBriteTM protocol for specific information)** | ||
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| Polar Body | 69°C x 8 min | 37°C x 4 hrs |
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| Uroepithelial | 73°C x 2 min | 39°C x 4 hrs-O/N |
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