FISH
The detection of genetic aberrations in malignant tissues with DNA Fluorescence in situ Hybridization (FISH) probe technology is a powerful means to diagnose and more efficiently treat a wide range of cancers. Abbott Molecular offers a comprehensive line of direct-labeled DNA probes for tumor assessment. Single and multi-color probe sets offer researchers and clinicians a variety of ways to identify chromosome or locus deletions, gains, or translocations that occur in cancers. These probes can be applied to a variety of sample types prepared for metaphase or interphase analysis.
The Vysis® FISH probes for oncology provide the following advantages:
DUAL COLOR, SINGLE FUSION, which is useful in detecting high percentages of cells possessing a specific chromosomal translocation. The DNA probe hybridization targets are located on one side of each of the two genetic breakpoints.
ES (Extra Signal), which reduces the frequency of normal cells exhibiting an abnormal FISH pattern due to the random co-localization of probe signals in a normal nucleus. One large probe spans one breakpoint, while the other probe flanks the breakpoint on the other gene.
DUAL COLOR, BREAK APART, which is useful in cases where there may be multiple translocation partners associated with a known genetic breakpoint. This labeling scheme features two differently colored probes that hybridize to targets on opposite sides of a breakpoint in one gene.
DUAL COLOR, DUAL FUSION, which greatly reduces the number of normal nuclei exhibiting abnormal signal patterns. The probe offers advantages in detecting low levels of nuclei possessing a simple balanced translocation. Large probes span two breakpoints on different chromosomes.
- Specific high-intensity signals without amplification and detection steps
- Low background for easy analysis
- Rapid, convenient, and easy-to-use assays
- Many probes designed for gene amplification detection include internal control probes
- Protocols that offer hybridization as quickly as four hours on the ThermoBrite® Denaturation and Hybridization Systems
- Solid tumor probes have been optimized for paraffin-embedded tissues
DUAL COLOR, SINGLE FUSION, which is useful in detecting high percentages of cells possessing a specific chromosomal translocation. The DNA probe hybridization targets are located on one side of each of the two genetic breakpoints.
ES (Extra Signal), which reduces the frequency of normal cells exhibiting an abnormal FISH pattern due to the random co-localization of probe signals in a normal nucleus. One large probe spans one breakpoint, while the other probe flanks the breakpoint on the other gene.
DUAL COLOR, BREAK APART, which is useful in cases where there may be multiple translocation partners associated with a known genetic breakpoint. This labeling scheme features two differently colored probes that hybridize to targets on opposite sides of a breakpoint in one gene.
DUAL COLOR, DUAL FUSION, which greatly reduces the number of normal nuclei exhibiting abnormal signal patterns. The probe offers advantages in detecting low levels of nuclei possessing a simple balanced translocation. Large probes span two breakpoints on different chromosomes.
