RealTime HCV Design



Development Philosophy
Today's clinical molecular diagnostics laboratory must have confidence in the quality of HCV patient results. As a result of our real-time PCR development philosophy, Abbott RealTime HCV is engineered to quantify all HCV genotypes (1—6) equally.

Accurate Quantification is dependent upon a combination of:
  • Primer Design
  • Probe Design
  • Cycling Conditions

Primers and Probe are Targeted to the 5´UTR Region of the HCV Genome
The target sequence for the Abbott RealTime HCV assay is in the 5´utr region of the HCV genome. This region is specific for HCV and is highly conserved. The primers are designed to hybridize to the 5´utr region with the fewest possible mismatches among HCV genotypes 1-6.

Single Stranded Linear Probe Design




 

 

 

 

 

 

 

 

 

 

 

Cycling Conditions:  Low Temperature Read Cycles


The cycling conditions for Abbott RealTime HCV encompass a low temperature read cycle. This read cycle allows the probe to tolerate mismatches more effectively than a probe required to bind during the extension phase.
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