Cystic Fibrosis V.3.0 Description

Background
Cystic Fibrosis is an inherited genetic disorder that affects children and young adults. It is caused by a number of genetic mutations in the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) gene. The American College of Obstetricians and Gynecologists currently recommends that couples planning a pregnancy or seeking prenatal care be screened for cystic fibrosis gene mutations.

For a list of mutations please click here.

The  CFTR gene comprises 27 exons and spans ~250 kb on the long arm of chromosome 7.

Since the cloning of the CFTR gene, more than 1300 mutations have been identified.  Mutations in the CFTR protein affect the ability of epithelial cells to secrete chloride ions in response to cyclic AMP.

Benefits

• Quality Controlled Reagents
• High Specificity
• Simple to use
• Cost Effective

Technology Description
The Abbott Molecular CF v3.0 reagents are based on Oligonucleotide Ligation Assay (OLA) technology. This technology combines target specific PCR amplification with OLA probes to produce allele specific fragments which can then be analyzed by electrophoretic Sequence Coded Separation (SCS).

OLA exploits the ability of DNA ligases to join adjacent nucleotides covalently only when they perfectly complement a single-stranded DNA template.